If the first sterilization of H. pylori is unsuccessful, a second radical treatment can be carried out, but it is necessary to follow the doctor’s instructions to use the full dose and full course of medication. The first unsuccessful sterilization of H. pylori may be due to antibiotic resistance or insufficient course of medication. The second radical treatment can be carried out, it is best to do a drug sensitivity test, choose sensitive antibiotics for treatment, to prevent the cause of drug resistance and lead to therapeutic failure. If the 1st application of amoxicillin and clarithromycin does not cure, it is necessary to have an interval of more than two weeks and treat with two other antibiotics, such as minocycline and tinidazole, combined with colloidal bismuth pectin and rabeprazole. If the 1st sterilization of H. pylori is unsuccessful, it is recommended to seek medical attention and be evaluated by a physician for a 2nd sterilization treatment.