Chorionic villus aspiration, also known as chorionic villus puncture, is commonly used clinically to diagnose fetal chromosomal abnormalities, neural tube defects, and other inherited metabolic disorders. Chorionic villus aspiration is performed between 11 and 14 weeks of pregnancy. 1. Indications for chorionic villus puncture: including NT ultrasound suggestive of thickening, hydatid cystic tumor in the neck, nasal bone not shown, fluid dark area in the abdomen, and abnormal cardiac structure. 2. Timing of puncture: Because amniocentesis in the middle of pregnancy is late if fetal chromosomal abnormalities are found and then intervention is performed. Therefore, transabdominal chorionic villus aspiration biopsy from 11 weeks to 14 weeks of pregnancy can advance the timing of prenatal diagnosis. 3. Puncture method: under ultrasound guidance, the puncture needle is inserted into the chorionic tissue of the placenta through the abdominal wall and uterus avoiding the amniotic cavity. Chorionic villus aspiration should be performed in an authorized medical institution, where the doctor will determine the timing of the procedure and perform it professionally according to the specific situation.