Nucleic acid testing can be performed twice a day, but twice a day is not very meaningful. Generally, the nucleic acid test is used to screen for patients infected with novel coronavirus pneumonia and is a non-invasive, non-invasive, non-invasive test that is performed by sampling a nasal or pharyngeal swab. Performing the nucleic acid test twice a day does not harm the body, but only causes a certain degree of discomfort, and there is no rule about how many times a day it should be done, so it can be done twice a day. According to the requirements of the epidemic, some people need to repeat the test several times for screening, but it is not meaningful to do the nucleic acid test twice in one day, and it causes a waste of epidemic prevention resources. In short, the nucleic acid test can be done twice a day, but it may bring discomfort and waste epidemic prevention resources, and it is meaningless. Tip: In this article, “new coronavirus pneumonia” and “new coronavirus pneumonia” have been renamed to “new coronavirus infection” on December 26, 2022, as announced by the National Health Commission.