Micro sperm extraction can be done a second time. Microsperm extraction is an assisted reproductive technique that involves extracting sperm from the testes of patients with non-obstructive azoospermia under a surgical microscope through microsurgical techniques. The indications include: patients with Kirschner’s syndrome, patients after cryptorchidism, patients with Y-chromosome deletion, patients with non-obstructive azoospermia whose testes are too small for testicular biopsy, and patients in whom spermatozoa are not found on testicular biopsy. Micro sperm retrieval is less creative, if you need to retrieve sperm again after the first retrieval due to some reasons, you can also have a second micro sperm retrieval, because micro sperm retrieval, the damage to blood vessels is smaller, the damage to the testicles is smaller, and the recovery after the operation is faster, so you can have a second retrieval. If you want to have micro sperm extraction operation, please visit a professional organization.