The diagnosis of brucellosis needs to be judged at three levels: epidemiologic investigation, clinical manifestations, and laboratory tests, which patients cannot judge by themselves. Brucellosis, also called brucellosis, is a natural epidemic disease caused by Brucella, clinically characterized by prolonged fever, excessive sweating, malaise, muscle and joint pains, and enlargement of the liver, spleen and lymph nodes. The main infectious sources of brucellosis are poultry and wild animals. It can be transmitted by skin and mucous membrane contact, and also through the digestive and respiratory tracts. Patients often have contact with diseased poultry and wildlife. Laboratory tests to confirm the diagnosis of brucellosis mainly include pathogenetic tests and immunologic tests. Pathogenetic tests can take specimens of the patient’s blood, bone marrow, cerebrospinal fluid, etc., and culture them, and the diagnosis can be confirmed if Brucella bacteria are cultured. Immunologic tests determine the presence or absence of infection by detecting antibodies to Brucella. The diagnosis of brucellosis needs to be made by a specialist in conjunction with the epidemiological investigation, clinical manifestations, and laboratory tests of the patient, and the patient cannot make the judgment on his/her own.