The time to turn negative for two plus urine protein is related to the type of disease, the severity of the disease and the patient’s own condition, and needs to be analyzed on a case-by-case basis. In case of primary glomerular disease with microscopic lesions, urine protein with two plus signs may turn negative in about 2 weeks after using sufficient amount of hormones. In case of membranous nephropathy, the urine protein will be reduced 4-8 weeks after the application of glucocorticoids combined with immunosuppressive therapy, and it will take about six months for the continued conversion to negative. In case of IgA nephropathy and focal segmental sclerosis, urine protein with two plus signs is difficult to turn negative even if both Chinese and Western drugs are used. Many chronic kidney disease proteinuria may not turn negative for life, and the turn negative may also relapse. If a patient has two plus urine protein, it is recommended to go to the nephrology department of a regular hospital in time to be examined by a professional doctor, and after a clear diagnosis, an individualized treatment plan will be made.