1.Seminal fluid collection and examination generally requires 3~7 days of abstinence, with exceptions for special cases; 2.Seminal fluid is generally collected by masturbation method, if there is difficulty in collecting semen, the doctor must be informed; 3.Clean hands and external genitalia; 4.The semen is collected in the seminal fluid collection cup by masturbation method, the ejaculation process is required to be thorough and the specimen is collected without omission, otherwise the test result and treatment effect are affected; 5.Don’t touch the inner wall of the seminal fluid collection cup or make the pubic hair fall into it when collecting semen.