Nucleic acid testing can be done in the evening in regular hospitals. However, emergency nucleic acid testing is only available for patients who require emergency care. Nucleic acid testing is not available for non-emergency patients, either during the day or at night. Emergency care is a valuable medical resource that should not be wasted. There are many types of nucleic acid tests, including nucleic acid tests for the new coronavirus, nucleic acid tests for the hepatitis B virus, and nucleic acid tests for the human papillomavirus (HPV). Different nucleic acid tests are performed in different ways, such as blood sampling for hepatitis B virus nucleic acid testing, tissue smear for HPV nucleic acid testing, and throat secretions for new coronavirus nucleic acid testing, etc. In order for nucleic acid testing to be performed smoothly, some precautions need to be understood first. Before performing the nucleic acid test, it is recommended to wear looser clothes for collection. To avoid discomfort during the nucleic acid test, it is recommended to perform it two hours after meals or on an empty stomach. It is recommended not to smoke, drink or chew betel nut within half an hour before sampling to avoid inaccurate test results. Hospital premises have a high flow of people, it is recommended that before the test must carry a good mask, maintain a safe distance of more than one meter from other testers, carry a good ID card and other documents, show the trip code in time, actively cooperate with the medical staff and follow the process of admission. Because each hospital system is different, if you are not a group of people who urgently need nucleic acid test results, you can make an appointment in advance, go to the local nucleic acid testing site or clinic for testing, and perform nucleic acid testing according to the appointment time to avoid the gathering of people.