Individualized therapy is playing an increasingly important role in cancer treatment. Individualized therapy refers to the detection of drug targets, pathways, metabolism and other targets in the patient’s body to assess the possible effects of drugs on the patient, improve the targeting and effectiveness of treatment, avoid adverse effects, reduce the risk and cost of treatment, and buy valuable time for the patient to heal. In short, individualized therapy is: using the right drug, prescribing the right dose, for the right person, at the right time. The principle of individualized targeted therapy is to exploit the molecular biological differences between tumor cells and normal cells for drug development, and the drugs act on specific targets of tumor cells, such as receptors, kinases and other proteins related to cell signaling, so as to specifically kill or inhibit tumor cells. Gene mutation testing for tumor patients can prospectively predict the effect of targeted therapy, provide good reference for doctors’ clinical use, and improve the accuracy and precision of targeted therapy. If patients have mutations, targeted therapy can be used decisively to bring positive treatment effect and hope for patients to live again. If the patient does not have the mutation, we can find other more suitable treatment for the patient, saving precious treatment time and avoiding unnecessary expenses.