For the sex hormone six test, it is clinically important to note that: 1. Blood should be drawn on an empty stomach; 2. Before the blood is drawn, it is important to sit still for 10-15 minutes and draw blood after resting. The main reason is that prolactin is affected by exercise, and after exercise can lead to an abnormal increase in prolactin, which will give the results an untrue reference value, so the blood should be drawn after resting. Sex hormone six test is mainly used in clinical practice for menstrual disorders, infertility, suspected premature ovarian failure, or in clinical practice, after ultrasound examination of bilateral ovaries with polycystic changes, to understand whether androgens are increased, these cases need to do the sex hormone six test.