According to the Technical Guide for Laboratory Testing of Neocoronavirus Pneumonia (7th edition), the main method for nucleic acid detection of neocoronavirus is the real-time fluorescent RT-PCR method, which targets the open reading frame 1ab (ORF1ab ) and the nucleocapsid protein (N) in the neocoronavirus genome. In general, to confirm a case as positive, the laboratory needs 2 targets of the neo-coronavirus (ORF1ab and N) to be positive at the same time in the same sample, i.e. a double gene positive (double positive). If the test results are positive for a single target of ORF1ab or N, it is called a single gene positive (single positive). In addition to the different number of detected targets, the test results also have different meanings for clinical purposes.1. Single positive: If a single positive appears, it is not possible to confirm whether the infection is new coronavirus, and it is necessary to take another sample for retesting and combine the results with epidemiological history, clinical manifestations, antibody testing, and imaging examinations to make a comprehensive determination. If the result is positive, active isolation treatment is required. If the result is negative, you should not take it lightly, do your daily protection, and report any abnormalities in time. 2. Double positive: If the result is double positive, it can be used as a basis for infection with the new coronavirus, but false positive cases arising from sampling errors need to be excluded. Therefore, further sampling of upper respiratory specimens (such as pharyngeal swabs, nasal swabs, etc.), lower respiratory specimens (such as respiratory aspirates, bronchial lavage fluid, alveolar lavage fluid, deep cough sputum, etc.), stool specimens, anal swabs, blood and serum specimens, etc. is usually performed to detect nucleic acids or antibodies in them, and to confirm infection with or without neo-coronavirus by combining epidemiological history, clinical manifestations, imaging examinations, etc. In the current situation of epidemic prevention and control, the government and corresponding institutions have very mature prevention and control measures, so people do not need to worry too much, do a good job of opening windows and ventilation, and avoid going to crowded places. Wear a mask when going out and avoid close contact with people with fever, cough and other respiratory symptoms. If you have fever, weakness, dry cough and other uncomfortable symptoms, you should go to the hospital promptly.