Mycoplasma and chlamydia infections in men often cause non-gonococcal urethritis because the human urethral mucosa is a good host for mycoplasma and chlamydia, which colonize the human urethra, thus causing a local inflammatory response, mainly manifested by symptoms such as increased urethral discharge, burning urine, and stinging urine. Mycoplasma and Chlamydia infections are transmitted by means of sexual contact, and infection with the above-mentioned pathogenic bacteria is easily caused through unclean sexual contact. For this condition, men should be actively diagnosed and treated to avoid further spread of the disease due to untimely treatment. For mycoplasma and chlamydia infections can be treated with doxycycline, azithromycin and daikonin, and sexual intercourse should be prohibited during treatment.