Hormone 6 test on the 3rd day of menstruation is the most accurate. The 2nd-3rd day of menstruation belongs to the early follicular stage, which is able to adequately respond to the function of the ovaries. As some women do not experience a significant drop in hormone levels during the luteal phase, the amount of bleeding on the 2nd day of menstruation is relatively low. It is recommended that the hormone six test be performed on the third day of menstruation by drawing blood from a vein at 9:00 a.m. on an empty stomach for maximum accuracy. If a woman has not been menstruating for a long time, the estrogen test can be used to diagnose the cause of the irregular menstruation. Fasting venous blood can be drawn at any time for a hormone 6 test, which is beneficial to the diagnosis of the disease. Disorders of the six hormones can affect normal menstruation and lead to menstrual disorders in the less severe cases, and can affect conception and lead to infertility in the more severe cases.