The most common case of sperm duct obstruction is a blockage of the vas deferens. The method of checking for vas deferens blockage can be determined by specific medical conditions. The simplest and easiest method is testicular tissue biopsy, which is the examination of a testicular biopsy. If the blood levels of follicle stimulating hormone and testosterone are normal and the testicular biopsy is normal, you can be sure that the vas deferens is blocked or that the vas deferens is congenitally underdeveloped. Otherwise, it may be a problem of spermatogenic function. Percutaneous epididymal fine needle aspiration for sperm extraction or percutaneous testicular fine needle aspiration for sperm extraction has the advantages of being minimally invasive and less painful, the aspiration process only takes a few tens of seconds, and no hospitalization is required. The biopsy can be done with the husband’s sperm if there is live sperm (ICSI). The remaining specimens tested at the time of biopsy can still be frozen. When a patient undergoes ICSI. If the recovered sperm is still viable, there is no need to perform another puncture for sperm extraction, which reduces the patient’s pain and protects the testes from re-injury. The number of sperm will drop significantly within a few weeks after the biopsy, even causing a temporary absence of sperm in the testes and in some cases causing an immune reaction; these effects generally take 3-4 months to return to the situation before the procedure, so it generally takes 3-4 months after the biopsy to do ICSI, during which the male partner should also take some drugs to help sperm growth. In conclusion, with the development of assisted reproductive technology, it is believed that more infertility patients will be brought to life.