Currently patients undergo three-generation IVF technology for screening of diseases mainly for the following categories: 1, carriers of chromosomal structural abnormalities, commonly chromosomal balanced translocation, Roche translocation, and chromosomal inversions; 2, there is currently a clear genetic diagnosis of a single-gene disease, such as thalassemia disease in Guangdong Province or the most common in the Zhongshan region, and the other is the Y chromosome microdeletion affecting the male azoospermia or severe oligo-spermatozoospermia, and this type of patients can also be through the three-generation IVF technology to select the female embryos, thereby blocking the transmission of the genetics of the genetic abnormality of the follower.