Physical examination – the physician examines the affected skeletal muscle for weakness and positive fatigue test (i.e., muscle intolerance soon after sustained contraction) Neostigmine test – the weakness of the affected skeletal muscle appears to improve significantly after 20 minutes of observation by intramuscular injection of neostigmine 1 mg, this test This test requires the physician to carefully observe the changes in the patient before and after the injection of neostigmine. Repetitive electrical stimulation – is the key to the diagnosis of the disease, but the sensitivity is too low for the oculomotor type, even if it is negative it still cannot exclude the oculomotor type of myasthenia gravis, but patients with the generalized type are usually found to be positive for low frequency stimulation attenuation, high frequency stimulation should be performed at the same time to exclude myasthenia gravis syndrome. Acetylcholine receptor antibody (AChR antibody) – is also an important basis for the diagnosis of the disease, but its specificity is greatly affected by the detection method, the specificity of radioimmunoassay is as high as 99%, which means that once the antibody is positive, the diagnosis of myasthenia gravis can be confirmed; however, the specificity of the enzyme-linked immunosorbent assay is poor, and domestic However, the specificity of the enzyme-linked immunosorbent assay is poor, and this method is basically used in China, so you should not blindly believe in the positive antibody result in China. The sensitivity of the antibody is greatly influenced by the type of myasthenia gravis, with a high positive rate for the systemic type and a low positive rate for the oculomotor type, and a negative antibody still does not exclude the disease.