A negative hepatitis B panel indicates that the body is not currently or previously infected with the hepatitis B virus, but a negative hepatitis B surface antibody also indicates that the body is not immune to the hepatitis B virus and is susceptible to hepatitis B virus infection. If a person has been previously vaccinated against hepatitis B and then has a full negative test, this reflects a failure of the hepatitis B vaccination. For those who are completely negative for hepatitis B, hepatitis B vaccination can be administered to make the body immune to the hepatitis B virus and provide protection. In addition, we can follow up the hepatitis B five items after vaccination to understand the success of the vaccination, and we can also follow up the hepatitis B antibody titers for a long time after vaccination to determine whether a booster vaccination is needed.