The locations of IUI injections include the uterine cavity, cervix, vagina, fallopian tubes and abdominal cavity. Artificial insemination (AI) is a technique in which sperm is injected non-coitally into a woman’s reproductive tract to fertilize her. Infertile couples with normally developing follicles, a normal range of motile sperm counts, a sound female reproductive tract structure, and at least one patent fallopian tube can undergo AI treatment. According to the insemination site can be divided into intrauterine insemination, intracervical insemination, intravaginal insemination, tubal insemination and direct transabdominal insemination. Clinically, the most common are intrauterine insemination and intracervical insemination. Intrauterine insemination (IUI) is usually performed on the day when the woman is expected to ovulate, usually after the follicle has grown to 1.8-2.0cm, and the semen is taken from the male partner, washed and optimized, and then injected into the woman’s uterine cavity through a catheter via the cervix. The specific method of artificial insemination and the location of the injection need to go to a qualified hospital, under the guidance of the doctor, to formulate an individualized treatment plan to carry out, not to choose on their own, to avoid affecting the conception rate.