The non-invasive DNA prenatal testing technology only requires venous blood from pregnant women, and uses new generation DNA sequencing technology to sequence free DNA fragments (including fetal free DNA) in maternal peripheral plasma, and the sequencing results are analyzed for bioinformatics, from which fetal genetic information can be obtained to detect whether the fetus has trisomy 21 (Down’s syndrome), trisomy 18 (Edward’s syndrome), trisomy 13 (Patau’s syndrome), and trisomy 21 (Down’s syndrome). The genetic information can be used to detect whether the fetus is suffering from the three major chromosomal disorders: trisomy 21 (Down’s syndrome), trisomy 18 (Edward’s syndrome) and trisomy 13 (Patau’s syndrome). Liu Ning, Genetics and Prenatal Diagnosis Center, First Affiliated Hospital of Zhengzhou University Non-invasive DNA prenatal testing is a safe, accurate, simple and rapid technique for detecting fetal chromosomal disorders. Non-invasive DNA prenatal testing technology for prenatal detection of fetal chromosomal aneuploidy diseases has the following advantages: 1. Safety: no trauma, no abortion, no risk of infection; 2. Accuracy: DNA level testing with an accuracy rate of over 99%; 3. Early pregnancy testing: testing can be done at 12 weeks of pregnancy or more; 4. Short testing cycle: testing report will be issued within 10 working days after blood collection. Non-invasive DNA prenatal testing technology is suitable for singleton pregnant women above 12 weeks of pregnancy, and the best testing time is recommended for 12-26 weeks. 1.Pregnant women aged 35 years or older; 2.Pregnant women with high risk of serological screening (Down’s syndrome screening); 3.Pregnant women who are virus carriers (such as hepatitis B virus carriers); 4.Pregnant women with a history of multiple miscarriages or children with chromosomal disorders; 5.Pregnant women who are pregnant with IVF or other precious children; 6.Pregnant women who are not suitable for invasive prenatal diagnosis due to placenta previa, low gestational sac or low amniotic fluid; 7.Pregnant women who are not suitable for invasive prenatal diagnosis due to placenta previa, low gestational sac or low amniotic fluid Pregnant women who are RH blood group negative; 8. Pregnant women who do not want to bear the risk of invasive prenatal diagnosis due to excessive psychological pressure.