ER, PR: ER and PR are present in normal breast epithelial cells, and when cells become cancerous, ER and PR are partially or completely absent. If the ER and/or PR are still retained, the growth and proliferation of the breast cancer cells are still regulated by endocrine control, which is called hormone-dependent breast cancer; if the ER and/or PR are absent, the growth and proliferation of the breast cancer cells are no longer regulated by endocrine control, which is called non-hormone-dependent breast cancer. C-erbB2 oncogene: It is lowly expressed in normal breast tissues, and its expression rate can be increased in breast cancer tissues. Its expression is positively correlated with breast cancer grade, lymph node metastasis and clinical stage, and the higher the expression rate, the worse the prognosis may be. P53 gene: Breast cancer cells with high p53 mutation rate have high proliferation viability, poor differentiation, high malignancy, aggressiveness and high lymph node metastasis rate. COX-2 (cyclooxygenase-2): The expression of COX-2 is present in breast cancer tissues, and COX-2 may be a useful indicator for clinical evaluation of patient prognosis and identification of patients at high risk of postoperative recurrence. Ki-67: correlates with the prognosis of breast cancer, especially in patients with negative lymph node metastases, and helps to determine whether to use adjuvant chemotherapy. E-cadherin: adhesion molecule, which can be used as a prognostic indicator in breast cancer. PS2: PS2 may be more useful than ER assay in predicting response to endocrine therapy, and PS2 expression is the best indicator of response to endocrine therapy in breast cancer. p63: p63 gene itself is an oncogene. p63 plays an important role in the occurrence and development of breast cancer; the test can provide the necessary theoretical basis for early diagnosis, timely treatment and prognosis judgment of breast cancer. Calponin: In normal, hyperplastic and atypical hyperplasia groups, almost all myoepithelial cells expressed p63, α-SMA and calponin, while all glandular epithelial cells were negative for the three antibodies; it is helpful to determine infiltrating carcinoma, carcinoma in situ and atypical hyperplasia. SMA (smooth muscle actin): smooth muscle actin is a reliable marker antibody. The disappearance of ME is a gradual process from normal breast tissue, benign lesions to carcinoma in situ, early infiltration and infiltrative carcinoma. Cyclin D1: High expression of Cyclin D1 may play an important role in the development and progression of human breast cancer. The clinical significance of high expression in breast cancer is that the expression of Cyclin D1 correlates with tumor size, TNM stage and axillary lymph node metastasis.