Non-small cell lung cancer antigen 3.53ng/ml is higher than the normal value range, which suggests that benign and malignant diseases of lungs, breast, gastrointestinal and other organs may be present. Non-small cell lung cancer antigen, also known as cytokeratin 19 fragment, CYFRA21-1, is mainly found in organs rich in epithelial tissues, such as lungs, mammary glands, intestines, bladder, etc. The normal value of CYFRA21-1 ranges from 0 to 3.3 ng/ml, and there are different degrees of elevation of CYFRA21-1 in benign and malignant lesions of the above organs. Non-small cell lung cancer antigen 3.53ng/ml is slightly higher than the normal range, which is mildly elevated and may be caused by benign diseases such as pneumonia, tuberculosis, cystitis, gynecology, gastrointestinal tract, etc., and there is a certain risk that it may be caused by malignant tumors such as non-small cell lung cancer, breast cancer and colorectal cancer. It is recommended that the patient should consult a specialist in a timely manner, make a comprehensive judgment by combining other tumor indicators and imaging tests, and follow the doctor’s instructions for further examination and treatment.