The five tests for hepatitis B include hepatitis B surface antigen, hepatitis B surface antibody, e antigen, e antibody, and core antibody. The surface antigen is the outer shell protein of the hepatitis B virus and is not infectious on its own, but its presence is often accompanied by the presence of the hepatitis B virus, so its positivity is a sign of having been infected with the hepatitis B virus. A positive serum test can usually be detected 2-6 months after infection with the virus, before the serum transaminases have risen. The vast majority of patients with acute hepatitis B can turn negative early in the course of the disease, but patients with chronic hepatitis B will continue to be positive. Surface antibodies, which are the body’s immune and protective antibodies against the hepatitis B virus, tend to appear positive during the recovery period. At the same time, the vast majority of people who receive the hepatitis B vaccine are also positive.　Positive e antigen is usually measured at the same time as, or within a few days of, a positive surface antigen after hepatitis B virus infection. e antibody positivity occurs several months after the antigen has turned negative. Core antibodies are usually detected in the serum 3-5 weeks after the appearance of surface antigen and before the onset of hepatitis B symptoms. Zhang Xiubin of the Department of Gastroenterology at Shandong Qianfo Mountain Hospital is commonly referred to as the five indicators of hepatitis B or “two-and-a-half” are the most commonly used serological tests for hepatitis B. The same patient can have different test results at different times. The analysis of the infectiousness of hepatitis B not only depends on whether it is “major triple-positive”, but also on whether the HBv-DNA is positive and the level of replication, in order to determine exactly how infectious hepatitis B is. The following is a brief description of the significance of the different combinations of the 5 indicators of hepatitis B. See the comparison table above. The term “major triple-positive” refers to row 1 of the table above, and “minor triple-positive” refers to row 2 of the table above.