I. Botulinum toxin biological wrinkle removal (Botox) 1, indications (1) facial power wrinkles (frown lines, crow’s feet, forehead lines, nasal back lines, nasolabial lines, perioral lines, etc.) (2) bite muscle hypertrophy 2, contraindications No obvious contraindications. Women should avoid menstruation. No recent fever, infectious diseases, etc. Botulinum toxin (Botox) was first used for strabismus treatment. Botox has become the first line of treatment for eye spasm, facial muscle spasm and neck muscle tension disorder; 2. In April 2002, it was approved by the U.S. Food and Drug Administration (FDA) and used in biological wrinkle removal; 4. Advantages: 1, fast results, immediate results, can quickly get rid of forehead lines, crow’s feet, between the eyebrows, nasal lines; 2, no pain, no swelling, does not affect work and study, that is, do that; 3, can be used repeatedly. After the effect disappears, it can be injected again. Fourth, the disadvantages: the main maintenance time is slightly short, about 9 months-1 year. The effect is not good for obvious sagging of the skin. The length of time to maintain the effect is related to the source of the drug, dosage, usage, injection physician’s skills, and the individual quality of the patient.