Hepatitis B major triplets can be converted into minor triplets, especially after antiviral treatment. If the major triplets are converted into minor triplets and the hepatitis B virus DNA turns negative, it indicates that the antiviral treatment is effective. In some patients, the above situation can also occur without the use of drugs, indicating that the degree of viral replication is reduced, the infectiousness is reduced, and the disease is less likely to develop. However, some patients are seen to turn from major triplet to minor triplet on their own, but the viral DNA is still positive, which is a mutation of the pre-C region of the virus, and the mutated strain of the virus may be more serious in the future and has a higher risk of developing liver cancer than the wild strain.