Non-gonococcal urethritis is a urinary tract infection caused by mycoplasma and chlamydia other than gonococcus, and is currently one of the most common sexually transmitted diseases of genitourinary infections in clinical practice. In recent years, the incidence of non-gonococcal urethritis has been increasing year by year in China and tops the list of sexually transmitted diseases in some Western countries, seriously endangering the physical and mental health of patients and affecting their health and quality of life. For the clinical treatment of non-gonococcal urethritis, there are many methods, but generally the course of treatment is long and easy to repeat, thus no unified standard has been formed, which brings obstacles to the clinical treatment of physicians. The clinician should therefore actively select a scientific and effective treatment for non-gonococcal urethritis. The main drugs for non-gonococcal urethritis are tetracycline and macrolide antibiotics, azithromycin as a new macrolide antibacterial drug, which can not only kill gram-positive and negative bacteria, but also inhibit the growth and reproduction of mycoplasma, chlamydia and syphilis spirochetes. Non-gonococcal urethritis is a urethritis caused by Chlamydia, Mycoplasma and some unknown pathogenic pathogens, mostly occurring in the sexually active period of young and middle-aged people. The incidence of non-gonococcal urethritis is increasing among the sexually transmitted diseases due to the openness of people’s mindset in modern society, and the incidence of social phenomena such as unclean sex and unpredictable sexual partners. The most important pathogens that cause non-gonococcal urethritis are Mycoplasma and Chlamydia trachomatis, Trichomonas vaginalis and Candida albicans, as well as the simple scarab virus. Mycoplasma is widely found in nature and is the smallest cellular microorganism that easily adheres to epithelial cells, especially on the surface of the genital tract, causing cellular damage and inflammation. Both men and women are susceptible to infection, with women mainly showing discomfort in the lower abdomen, abnormal menstruation, increased discharge, itchy urethra, cervical inflammation and erosion; men mainly showing enlarged, hard and painful epiglottis. If the infection is not treated in time, complications may even occur in serious cases. The actual fact is that there are many ways to treat non-gonococcal urethritis in the clinic, but the general method is long, easy to repeat, unable to remove the cause of the disease, which brings a great obstacle to the clinical treatment of physicians.