The skin wrinkle-reducing effect of biological wrinkle removal was discovered accidentally in 1986 by J.C., a professor of ophthalmology at the University of British Columbia School of Medicine in Vancouver, Canada, during the treatment of eyelid spasms, and in 1987 she began a study on the use of Botulinum toxin type A for skin wrinkle removal together with her husband, a professor of dermatology at the same department, and published the first research paper in 1992, becoming the founder of the use of Botulinum toxin type A for cosmetic purposes. She published her first research paper in 1992 and became the founder of Botulinum toxin type A for cosmetic use. Since then, after achieving satisfactory results, especially since this method has made Hollywood stars more youthful and beautiful, Botulinum toxin type A has become popular in the U.S. cosmetic field, with an annual growth rate of 142%, making other methods unparalleled, and has been previewed by authoritative experts as “the main method of cosmetic wrinkle removal in the 21st century”. The mechanism of biological wrinkle treatment is that it can act on peripheral motor nerve endings and neuromuscular junction, inhibit the release of acetylcholine from presynaptic membrane, which leads to muscle relaxation paralysis and treats muscle spasm and dystonia diseases. The appearance of facial wrinkles (such as interbrow lines, crow’s feet, forehead lines, nasal dorsal group, nasolabial folds, perioral lines and even broad neck lines), in addition to natural aging, is more importantly the excessive contraction of expression muscles, because the starting point or stopping point or both of these related muscles are connected to the skin, biological wrinkle removal indications: 25-50 years old, wrinkles are obvious, skin relaxation is not very Severe. Advantages: This method is effective in removing the headline, Kawasaki lines, crow’s feet at the corners of the eyes and nasolabial lines. No pain, no swelling, does not affect work and study, that is to do that is gone. It can quickly get rid of forehead lines, crow’s feet, frown lines, nasolabial lines. Shortage of biological wrinkle removal: The main maintenance time is slightly shorter, about 6 months-1 year. It is not effective for sagging and loose skin. The length of the maintenance effect is related to the source of the drug, dosage, usage, the technique of the injecting physician, and the individual quality of the patient.