The bacterial count for surgical hand disinfection monitoring should be ≤5 cfu/cm2. Surgical hand disinfection refers to the process in which medical personnel wash their hands with soap and running water before surgical procedures, and then use hand disinfectants to remove or kill the temporary resident bacteria and reduce the resident bacteria on the hands, while the bacterial count for surgical hand disinfection monitoring should be ≤5cfu/cm2. Surgical hand disinfection sampling method is the examinee five fingers together, with a corresponding neutralizing agent soaked in sterile eluent cotton swabs in both hands from the root of the finger surface to the end of the finger back and forth to rub twice, a hand coated with an area of about 30 square centimeters, the rubbing process should be at the same time to rotate cotton swabs, and then cotton swabs to contact with the operator’s part of the cut off into the 10 ml of the corresponding neutralizing agent in the test tube of the sterile eluent, and then sent for testing in a timely manner. It is promptly sent for testing. Since surgical hand disinfection is part of the code of practice for hand hygiene for medical staff, it is recommended that medical staff should wash and disinfect their hands before surgery.