DNA sequencing, also known as DNA sequencing, refers to the process of determining the order of the bases (ATGC order) of deoxyribonucleic acid (DNA) extracted from samples by sequencing equipment, so as to decipher the genetic code of DNA and provide guidance for life science research, clinical diagnosis and treatment. Genome sequencing can be roughly divided into six steps: DNA extraction, DNA fragmentation, DNA library construction, DNA amplification, sequencing, and analysis of sequencing results. Deoxyribonucleic acid DNA sequencing is used to examine and determine the nucleotide sequence of specific DNA fragments, and is of great value in the field of genetic disease detection. It is used in the field of disease screening and genetic disease detection to predict disease risk and regression, assist in disease typing and diagnosis, and guide the use of medication.