The diagnosis of prostate cancer also depends on prostate puncture biopsy. For any patient who suspects prostate cancer, timely systematic prostate puncture biopsy is an important step to confirm the diagnosis of prostate cancer at an early stage, and some people even undergo multiple puncture biopsies to confirm the diagnosis. Nowadays, a 12-stitch systematic puncture method with ultrasound guidance is usually used to improve the accuracy of the puncture. However, it is worth mentioning that even in patients with negative puncture results, the alarm should not be completely lifted and vigilance relaxed, but must be reviewed promptly and, if necessary, the puncture should be repeated to prevent a missed diagnosis. For more unusual puncture results such as PIN/ASAP, it is even more important to be vigilant and repeat the puncture in a timely manner. Many people refuse puncture because they worry that once it is really cancer, puncture will lead to cancer cell metastasis and stimulate the growth of cancer. There is no scientific basis for this, and clinical studies with bulk data have ruled out this possibility.