There are more tests for male infertility, and the standards implemented by various medical institutions are not uniform, and there is some blindness in the selection of methods, resulting in a waste of time and economy. In order to reduce the occurrence of these problems, the common tests for infertility and their scope of application are briefly described below for reference only. 1, physical examination: general (development, nutritional status) and genital (testes, epididymis, vas deferens, spermatic vein, penis, prepuce and urethra) examination, applicable to each patient; 2, semen analysis: mainly sperm density, motility and morphology, applicable to each patient; 3, serum sex hormones: mainly testosterone (T) and follicle stimulating hormone (FSH), applicable to azoospermia, oligospermia and 4. Anti-seminal antibodies: mainly anti-seminal antibodies in seminal plasma, sperm surface and cervical mucus of the female partner, anti-seminal antibodies in serum are not significant. Applicable to poor sperm vitality, more sperm agglutination, or unexplained infertility; 5. Chromosomal analysis: applicable to azoospermia, extreme oligospermia, primary small testes and unexplained miscarriage and fetal abortion of the female partner; 6. Seminal plasma biochemical analysis: most of the clinical significance is not clear, only a-glycosidase and fructose, reflecting the function and patency of the epididymis and seminal vesicles respectively, applicable to suspected sperm duct obstruction; 7. Examination of prostatitis: most prostatitis has no obvious and long-lasting effect on fertility, and special examination is generally unnecessary; 8. Ultrasound: scrotum and contents, for those suspected of testicular epididymal or spermatic cord lesions; prostate, seminal vesicle glands and ejaculatory ducts (done transrectally), for those with azoospermia, extreme oligospermia and low semen volume; 9. Seminal vesicle and vasography: invasive and risky, for those suspected of ejaculatory ducts or vas deferens 10. Testicular biopsy (puncture or incision): invasive and risky, suitable for azoospermia, especially for those with large testicular volume, FSH not significantly elevated, and ready for IVF.