Hepatitis B immune globulin is also effective when given after 24 hours, but the protective effect is diminished. Hepatitis B Immune Globulin is extracted from the blood of healthy people, and it contains very high titers of hepatitis B surface antibodies that work to neutralize the hepatitis B virus, and it is indicated for people who have experienced exposure to the hepatitis B virus. Hepatitis B immunoglobulin is recommended within 12 hours for newborns of hepatitis B surface antigen positive mothers, or for people who have had hepatitis B exposure. If, for personal reasons, the vaccination is given after 24 hours, it is also protective, but the protection is diminished. For healthy individuals (hepatitis B surface antigen negative), hepatitis B vaccination should be completed at a vaccination site when hepatitis B surface antibodies are lacking in order to obtain hepatitis B surface antibodies, which will protect against hepatitis B infection.