Theoretically, HBsAg and HBV-DNA can be detected in the milk of HBV-infected pregnant women, and the transmission of HBV from mother to infant may occur through nipple cracking, excessive sucking or even nipple biting by the infant, but there is a lack of evidence-based medical evidence for this. However, the evidence proves that even without immunoprophylaxis for newborns, the prevalence of HBV infection in breastfed and artificially fed newborns is almost equal; even if the pregnant woman is HBeAg positive, breastfeeding does not increase the risk of HBV infection in newborns. After taking regular preventive measures for newborns, regardless of whether the pregnant women are HBeAg positive, their newborns can be directly breastfed without testing HBV-DNA in breast milk.