For many patients with azoospermia, a clear diagnosis is the first step that needs to be taken. At present, the most common tests need the following: 1, semen examination after centrifugal sedimentation, if the simple smear is easy to misdiagnose, this test is necessary, because our clinical experience has proved that there are individual patients who were diagnosed with azoospermia after just two semen routine, and found sperm after our centrifugal treatment, in addition to the need to apply more smears and find more fields of view during the semen laboratory test, to carefully recognize the responsible. 2, sex hormone examination, generally do sex hormone six, including follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estradiol (E2), progesterone (P), prolactin (PRL), if FSH is significantly elevated, generally considered greater than twice the normal testicular spermatogenic dysfunction, can not do the third test; 3, testicular biopsy, mainly do testicular histology At present, many units carry out multi-point testicular puncture for tissue suspension examination, which used to be considered as the final diagnosis of azoospermia, but due to the differences in pathological examination methods, the examination results also need to be carefully considered, for example, the sampling site may not necessarily reflect testicular function comprehensively; 4, vasectomy, although there are views that doing seminal plasma biochemical examinations such as: fructose, alpha-glucosidase, carnitine, etc. Vasectomy is still the most reliable diagnostic method to clarify obstructive azoospermia.