Spina bifida, especially occult spina bifida, is often difficult to detect by prenatal ultrasound, and most occult spina bifida may not have any clinical symptoms, but some occult spina bifida has a poor prognosis because of the clinical syndrome of lower extremity motor and sensory, urinary, and defecation dysfunction, known as spinal cord embolism syndrome, which plagues many hospitals and children with occult spina bifida with spinal cord embolism syndrome. In the past three years, we have conducted prenatal ultrasound studies of spinal-related disorders and have found that prenatal ultrasound routinely locates the fetal spinal cone and provides valuable information for the diagnosis and differential diagnosis of spina bifida with spinal cord embolism and other related disorders in the spinal region. In the past three years, we have diagnosed three cases of fetal recessive spina bifida with spinal cord embolism, two cases of lipoma formation in the lumbosacral canal, two cases of caudal dysplasia syndrome, one case of spinal cyst in the lumbosacral canal, four cases of sacrococcygeal teratoma, and nine cases of overt spina bifida with spinal cord embolism. The spinal cord is equal in length to the spinal canal at the beginning of fetal development, after which the spinal cord grows slower than the spinal column, the spinal cord moves cephalad, and the spinal cord cone is located between the L2~3 gap at birth. Our study found that the location of the spinal cord cones in normal fetuses above 20 weeks of gestation are located above the level of the third lumbar vertebra. If the location of the spinal cord cones is below the level of the fourth lumbar vertebra, careful examination should be performed to clarify the presence of lumbosacral spina bifida or other diseases in the lumbosacral canal to provide valuable information for prenatal consultation and eugenics.