Old waking up from 3:00 am to 4:00 am and discomfort in the liver area may be related to diseases of the hepatobiliary system, in addition, it can also be seen in pleural and gastrointestinal diseases. 1. Liver diseases: such as viral hepatitis, cirrhosis, fatty liver and other diseases cause inflammation and edema of liver cells, the liver increases in size, pulling the nerves on the peritoneum, and discomfort in the liver area occurs. At night, the vagus nerve is excited, so the pain phenomenon may be more obvious. 2. Gallbladder diseases: such as cholecystitis, gallstones and other diseases, at night the vagus nerve excitation, bile secretion increases, which can cause spasm of the gallbladder or bile ducts, and even biliary colic. 3. pleural and gastrointestinal diseases: such as pleurisy, duodenal ulcer and other diseases, because of its proximity to the liver region, manifested as pain in the liver region. In short, the appearance of 3:00 to 4:00 a.m. old wake up and liver discomfort and other manifestations, should promptly seek medical attention, clear diagnosis, targeted treatment.