With the opening of the two-child policy, more and more couples came to the hospital for pre-conception physical examination, many couples are doubtful about the hand of mycoplasma, chlamydia examination, some patients themselves do not have any clinical symptoms, personal aspects are also clean, how can be infected with mycoplasma chlamydia it? Some couples even fall into each other’s suspicion and speculation, is not the other infection infected to their own it, seriously affecting the harmony of the couple. At present, there is some controversy in medical science regarding the diagnosis and treatment of positive mycoplasma and chlamydia tests. It causes abortion and is mainly transmitted through sexual contact. The PCR method has a sensitivity of more than 99%, which is the most sensitive of all methods, but I believe that the method is prone to false positives, and the isolation rate of Mycoplasma urealyticum in the urethra of normal men is 34% in foreign countries, while the rate of healthy people in China is 10.5%. Therefore, Mycoplasma urealyticum is a conditionally pathogenic bacterium, and detection is not equivalent to causing disease. So, some of you may ask, can the antigen or antibody of Chlamydia trachomatis and Mycoplasma trachomatis be detected by blood sampling? The answer is no. Chlamydia trachomatis invades and multiplies in the columnar epithelial cells, first forming foci of infection in the urethra and spreading along the genitourinary tract. Because of the tissue-selective (epitheliophilic) nature of these pathogens, it is not meaningful to detect antigens of Mycoplasma and Chlamydia trachomatis in the blood, so clinical testing of the above pathogens through blood specimens is not recommended. Is the method of direct microscopic examination of pathogens by means of stained smears reliable? The answer is still no. At present, clinical Chlamydia trachomatis inclusion bodies can only be used for conjunctival specimens and are not sensitive to specimens from other sites, while mycoplasma morphology and size are indistinguishable from other particles in tissue cells and therefore have little significance. The cell culture method is currently the most sensitive and specific method for detecting Chlamydia trachomatis infection, and the mycoplasma isolation culture method is the most reliable pathogenic examination method. Finally, as for the criteria for determining cure, many couples are still positive for DNA by PCR after treatment, are they still infected? The current criteria for judging the cure is that the patient’s conscious symptoms disappear, no discharge from the urethra, no white blood cells in the urine sediment, and generally no culture is done.