A negative IgM and IgG test indicates no infection and pregnancy is possible. If the initial infection occurs early in pregnancy, the fetus is at high risk and vaccination may be considered; there is currently a vaccine for rubella. A positive IgG indicates a previous infection and pregnancy is also possible. If IgG is negative and IgM is positive, acute infection is considered, or false positive. For those who are pregnant, it is important to determine if they are infected. If both antibody tests are positive, it may be an acute infection for Toxoplasma gondii and a late infection for other viruses. There is a lack of uniform quality control standards for antibody screening, and a variety of viral ELISA test kits are available, the sensitivity and specificity of which are unclear. And the false-positive and false-negative rates of the test are relatively high. The rate of Toxoplasma gondii infection in China is extremely low, so the significance of Toxoplasma gondii testing is not significant. In addition, there is no recurrence of rubella virus. Most women of childbearing age already have rubella IgG antibodies in their bodies, so they will not be infected during pregnancy and the incidence of congenital rubella syndrome due to rubella is extremely low. Rubella virus may not be screened for prior to pregnancy. Herpes simplex virus screening must be well typed. Most people have been infected with herpes simplex virus type 1 and have antibodies in their bodies. Herpes simplex virus does not cause fetal infection as long as there are no active lesions on the external genitalia. The herpes type 2 virus requires attention to the presence of active lesions on the external genitalia during the perinatal period, as active lesions during the perinatal period can easily lead to infection of the newborn. Cytomegalovirus has a high rate of infection, with 90% of women of childbearing age in Beijing having been infected prior to pregnancy. Cytomegalovirus is latent in the body and can be easily activated once the body’s immunity decreases, but its impact on the fetus is very weak after activation. Therefore, we need to pay attention to cytomegalovirus, but the need to do dynamic surveillance is still open to discussion.