1, abstinence time 48 hours to 7 days, generally 3 to 5 days is appropriate. 2, it is best to wash the glans at home before taking sperm, wash and dry your hands before taking sperm, avoid soiling the sperm cup with hand sanitizer and water, and avoid dropping foreign objects into the sperm cup (clothing cotton fibers, pubic hair, etc.). 3. It is best to urinate and empty the bladder before sperm extraction. 4. It is best to have no significant physical discomfort for 1 week before sperm retrieval, no history of special medication and poor lifestyle, etc. 5.Sperm retrieval should preferably be done by masturbation. If you have difficulty in retrieving sperm, please inform the obligatory staff in advance. 6. Those who retain semen at home should avoid using the interrupted sexual intercourse method, and should not use condoms to retain semen on their own, and should keep it at 20℃ to 40℃ during semen delivery, avoid violent shaking, and preferably send it for examination within 30 minutes. 7.Collect semen as complete as possible to avoid semen leakage outside the semen collection cup. 8.Pick up the report in room 7 or 11 on the 12th floor of Outpatient Building 1 after 2 hours of semen routine. 9. The report will be issued 5 days after the sperm malformation rate test, spermatogenic cytology test, seminal plasma anti-sperm antibody (MAR), sperm viability test, semen leukocyte test, seminal plasma biochemistry (fructose, neutral a-glycosidase, acid phosphatase) and other tests are performed.