Infertility is often the result of a variety of factors affecting both partners, and patients seen in the infertility clinic need to be carefully screened for causes through multiple examinations. First, the general history taking, physical examination and various assessments of the infertile couple. Second, routine semen analysis of the male partner is the primary test for infertility and is performed at the hospital within 3-7 days of abstinence. The test will be performed according to the WHO 5th edition standards, and for abnormal results, 2-3 retests will be performed to confirm. Secondary infertility also needs to be examined. III. Related examinations: including general physical examination, gynecological examination, vaginal ultrasound and special examination for infertility. (The doctor will pay attention to the mental state, general development and nutritional status of the patient, the development of secondary sexual characteristics including breast development, the presence or absence of overflowing breast, fat distribution, hair growth, masculinity, and physical variation or skin pigmentation abnormalities caused by endocrine disorders of the pituitary, adrenal, and thyroid glands, etc. 1.Body mass index (BMI): It is a world-recognized grading method to assess the degree of obesity, and its formula is BMI=weight (kg)/(height)2(m), and the normal range is 18.5-25kg/m2. 2.Waist-to-hip ratio (WHR): It is the ratio of waist/hip circumference, and the normal range should be <0.85, which is an important indicator to determine central obesity. As excessive waist fat can destroy insulin system and lead to diabetes, hypertension, hyperlipidemia and other diseases, and also lead to liver hypertrophy and affect liver function, thus patients should be encouraged to lose weight before treatment and treated after 15% weight loss, which can improve the treatment effect. 3, history of tuberculosis: most patients lack clear symptoms and positive signs, so when there is primary infertility, scanty menstruation or amenorrhea; unmarried women with low fever, night sweats, pelvic inflammatory disease or ascites; previous history of contact with tuberculosis or I had tuberculosis, pleurisy, intestinal tuberculosis should be considered the possibility of reproductive tuberculosis, the following tests are routinely performed: ① PPD test: is a highly sensitive and specific test. A positive test reaction is of great value in the diagnosis of tuberculosis infection, but it cannot diagnose the presence of tuberculosis in the lungs or determine the nature of the lesion. ②Serum-interferon: It is used for the diagnosis of latent infection of tuberculosis bacilli. ③Frontal and lateral chest radiographs or CT scan: ④Pelvic X-ray or CT scan: finding isolated calcified spots suggests a previous pelvic lymphatic tuberculosis lesion. ⑤ Blood sedimentation and anti-tuberculosis antibody: poor sensitivity and specificity, although widely used in clinical practice, but of little diagnostic significance. 4. Thyroid examination: to check for enlargement of the thyroid gland and to assess thyroid function, the main reason being that certain thyroid disorders interfere with normal ovarian function. (ii) Gynecologic examination The first examination must be performed to detect some obvious congenital genital anomalies, such as congenital absence of vagina, longitudinal vagina, double cervix and double uterus, etc. (c) Vaginal ultrasound is a common clinical examination method in reproductive medicine. It is especially clear for observation of endometrium and ovaries, especially for endometrial cancer, uterine fibroids, pelvic masses, follicle monitoring, early ectopic pregnancy, etc. The images are more clearly displayed. (D) Special infertility tests 1. Serum sex hormone measurement: Under normal circumstances, serum levels of various hormones are measured on the 2nd-5th day of the menstrual cycle in fasting to obtain information on the functional status of the ovaries and their influencing links, and to identify ovarian or pituitary ovulation disorders and amenorrhea. 2. Hysterosalpingography: Infertility caused by tubal disease accounts for 1/3 of female infertility, therefore, normal structure and function of the fallopian tubes is a necessary condition for normal pregnancy, and accurate evaluation of the structure and function of the fallopian tubes is a key part of the diagnosis and treatment of female infertility. Hysterosalpingography is the most commonly used test to find out whether the fallopian tubes are patent and the degree of patency and the specific site of obstruction. It is usually performed within 3-7 days after menstruation and without acute inflammation of the reproductive system. 3.Endometrial scraping: The purpose is to scrape the uterine cavity contents for pathological examination to assist in diagnosis. 4. Immunological examination: Some patients with primary infertility are found to have adverse immune reactions such as anti-sperm antibodies, anti-ovarian antibodies and anti-hyaline antibodies through testing, and such immune reactions can kill sperm or inhibit the union of sperm and egg, causing infertility, in addition to indirect understanding through post-coital test and in vitro sperm penetration test. 5. Hysteroscopy: Hysteroscopy can not only determine the site, size, appearance and scope of the presence of the lesion, but also make a detailed observation of the tissue structure on the surface of the lesion, and take the material or position the uterus for scraping under direct vision, which greatly improves the accuracy of the diagnosis of the disease in the uterine cavity and updates, develops and makes up for the deficiencies of traditional treatment methods. It is usually performed within 3-7 days after menstrual cleansing.