I. Overview Mycoplasma usually resides in the respiratory and genital tract mucosa, is a unique group of microorganisms, easy to form branching mycelium and named mycoplasma. They are the smallest known free-living microorganisms that contain both DNA and RNA, and are distinguished from bacteria by their absence of a cell wall. They can grow in cell-free medium and are therefore different from viruses. Mycoplasma urealyticum and Mycoplasma histolytica are common genital microorganisms, and in most women the presence of UU and MH does not cause significant adverse consequences. However, studies in the last two decades have found their association with a variety of clinical conditions, spontaneous abortion and stillbirth, low birth weight and preterm birth, chorioamnionitis, intra-amniotic infection, premature rupture of membranes, postpartum infection, pyelonephritis, inflammatory pelvic disease, and infertility. Laboratory diagnosis Commonly used methods include: mycoplasma culture (the best means to confirm the diagnosis); spot immunoconjugation test; indirect hemagglutination assay (IHA) and metabolic inhibition assay (M IT) for mycoplasma antibody detection with high specificity and sensitivity; enzyme-linked immunosorbent assay; polymerase chain reaction (PCR) technique. Mycoplasma culture requires special techniques and cannot be performed in most hospitals. Its serological technique is usually used only as a research tool, and ELISA is used to detect specific groups of antibodies, which are significantly elevated only to indicate a recent infection, but not to determine the site of infection. The metabolic activity of mycoplasma can be used to detect their growth in the medium. uU breaks down urea to produce ammonia and MH metabolizes arginine to ammonia, both of which raise the pH of the medium. Third, specimen collection women for cervical secretions, first swab the excess mucus from the cervical opening, then use a sampling swab into the cervical canal 1 to 2 cm, stay at least 30s rotate 1 to 2 circles, remove into a sterile test tube and immediately sent for testing. Reagents and methods Currently, reagents are mostly used in kits. The culture and drug sensitivity identification were carried out strictly according to the instructions of the kit. The experimental results show that the UU hole becomes red for UU growth, MH hole becomes red for MH growth, and both holes become red for UU and MH growth. The reddening of both wells indicated that the added drug had no inhibitory effect (drug resistance), while the reddening of both wells indicated that the added drug had inhibitory effect (sensitivity), and the reddening of the front hole was moderate sensitivity.