1.Basic sex hormone level measurement: including FSH, LH, E2, fasting from 10:00 p.m. on the previous day after the 2nd-4th day of menstruation; 2.Luteal phase hormone level measurement: blood is taken on an empty stomach on the 7th-8th day after ovulation (or elevated basal body temperature). If the menstrual cycle is normally regular, the 28-day cycle is equivalent to the 21st-22nd day of the menstrual cycle; 3.Pituitary prolactin measurement: it is not related to the menstrual cycle and can be measured at any time, but if the results are not normal, you should choose the standard time to take blood, and eat a moderate amount of carbohydrates for breakfast, and abstain from eating fat and meat and eggs; take blood between 10:00 am and 2:00 pm; sit still for half an hour before taking blood, but stay awake. 4. Ovulation hormone levels should be measured on an empty stomach after follicle maturation (18-20mm in diameter); 5. Testosterone levels are not related to the menstrual cycle and can be obtained at any time on an empty stomach.