Rotavirus infection is widespread and almost all children under 5 years of age have had at least one rotavirus infection, which can be recurrent; it is an important pathogen of nosocomial infections in the gastrointestinal tract, with a high incidence in the fall and winter. Most infections are due to direct or indirect contact with the patient, mainly fecal-oral transmission, but respiratory transmission is also possible. Duration of inoculation: 1-3 days, i.e., 1-3 days between the appearance of clinical symptoms after infection with the virus. In patients with diarrhea, rotavirus is excreted in large quantities in the stool. At present, our hospital can diagnose rotavirus quickly through fecal rotavirus antigen testing. To prevent outbreaks or epidemics of hospital-acquired rotavirus infection, clinical departments should do the following: 1. Rapid diagnosis of fecal rotavirus antigen test for suspected patients, confirmed rotavirus infection or carriers (positive laboratory test, no obvious clinical manifestations of diarrhea) need to be contact isolation: bedside contact isolation (hanging contact isolation sign) or single room isolation can be chosen. The principle isolation cancellation time for the child’s stool rotavirus test negative until. 2, rotavirus contamination of the hands after 20 minutes still has a survival rate of 16.1%, so health care workers in contact with the child and the surrounding environment need to wear gloves, before milk feeding, before and after contact with the patient and before and after the operation, after taking off the gloves need to wash their hands with running water, especially after handling the child’s excrement (diaper) to immediately wash their hands with strict running water. Note that the rapid hand disinfection method containing ethanol is not ideal for rotavirus disinfection. 3.Environmental disinfection should be carried out by wiping or spraying with 1000mg/L effective chlorine preparation, and the wet disinfection time should not be less than 30 minutes. 4. When the excrement of the child contaminates the surrounding environment or objects, the contaminated surface should be immediately disinfected with disinfection wipes containing 1000mg/L active chlorine for 30 minutes, and the contaminated clothes should be soaked for 30 minutes before uniform cleaning and disinfection. 5.The medical waste generated by the children should be sealed and placed in the medical waste bucket and covered tightly, and the medical waste bucket should be cleaned and disinfected every day.