Indications of artificial insemination: 1, male infertility due to oligospermia, weak sperm, abnormal liquefaction, sexual dysfunction, genital malformation, etc.; 2, cervical factor infertility; 3, infertility due to reproductive tract malformation and psychological factors leading to inability to have sexual intercourse, etc.; 4, immune infertility; 5, unexplained infertility. Indications for in vitro fertilization-embryo transfer (first-generation IVF): (1) gamete transport disorders caused by various factors on the female side; (2) ovulation disorders; (3) endometriosis; (4) oligozoospermia on the male side; (5) unexplained infertility; (6) immune infertility. Indications for intracytoplasmic monosperm microinjection (second-generation in vitro fertilization): (1) The man’s three semen examinations at least two of the following criteria: (1) extreme oligozoospermia (<1 × 106 / ml), extreme weak spermatidosis (a + b < 1%) or extreme dyszoospermia (normal sperm < 1%); (2) severe oligozoospermia, weak and abnormal spermatidosis: density < 5 × 106 / ml, viability a+b<10%, normal spermatozoa <5% (WHO 5th edition <2%); (2) irreversible obstructive azoospermia; (3) spermatogenic dysfunction (excluding genetic defects); (4) immune infertility; (5) failure of in vitro fertilization (previous IVF insemination rate <25%); (6) spermatozoa acrosome abnormality; (7) need to carry out preimplantation embryo genetics examination.