Children want to urine test, parents for the first time to do this technical work, always afraid to receive the wrong, often ask the question of retention of urine, is summarized as follows: 1, urine routine, urine NAG enzyme, urine red blood cell morphology, urine test kidney damage complete set, urine test tubular function, urine test glomerular function: leave the first clean mid-morning urine 10ml. 2, urine release specimen: the first cup of clean mid-morning urine for the first specimen 10ml, and then drink water 300-500ml, and then drink 300-500ml of water, and after you have the urge to urinate, keep the second cup of middle urine as the second specimen 10ml, and send the two cups for examination at the same time. 3, 24 hours urine test four: the day 7 o’clock morning urine discarded, from the second urine to the next day 7 o’clock morning urine, record the amount of urine on the test sheet, mix and then leave 10ml sent for testing. 4.Upright test urine specimen: Empty the bladder before going to bed, do not move in the morning, urinate in front of the bed, and retain 10ml of the middle section of urine for upright front urine. Then let the patient stand with the heel 20cm away from the wall, in the shape of chest and concave waist for 20 minutes, and then move freely, and when there is an urge to urinate, then take 10ml of middle urine, which is the upright post urine, both urine specimens need to be sent for examination. 5, urine bacterial culture specimen: first of all, get the urine culture specimen bottle, clean the vulva, the morning directly to the clean middle urine to the test tube, the amount of about 3 to 5 ml, pay attention to absolute cleanliness, do not contaminate the mouth of the bottle, do not tilt. Please do not keep the specimen temporarily if the female patient is menstruating, and do not keep the urine specimen during the infusion process of the child because the result may be inaccurate.