Prediction of ovarian reserve capacity: 1, age: >35 years of age ovarian reserve capacity declines significantly, but there are individual differences; need to be combined with other indicators; 2, menstrual cycle: regular shortening of the menstrual cycle often indicates a decline in reserve capacity; 3, ovarian disease and surgical history of ovarian function; 4, sinusoidal follicle count: the whole ovary count of ≤ 5 or the most cut ≤ 3 suggests a decline in reserve; 5, basic FSH level: generally ≥ 15U / L; 6, basic E2 level: E2 > 80pg / ml on day 3 of menses, regardless of age and FSH level is already clear that it is the reserve decline; 5, basal FSH level: generally ≥15U/L, which is more significant; 6. Basal E2 level: E2>80pg/ml on the 3rd day of menstruation, regardless of age and FSH level, it is already clear that its fertility has declined. 7, Basic inhibitin and AMH level: inhibitB<80pg/ml or AMH<8pmol/l, suggesting ovarian hyporesponsiveness; 8, FSH/LH ratio: >3.6, specificity 95%, sensitivity 85%; 9, CC stimulation test: Measure the blood FSH level on the 3rd day of menstruation, and then take 100mg of CC orally on the 5th-9th days, and measure FSH again on the 10th day of menstruation. FSH is measured on day 10, if FSH level is elevated (>26U/L), the reserve is decreased; 10. History of previous COH. Diagnostic criteria of ovarian hyporesponsiveness: At present, most scholars consider that follicles that have developed to the mature stage, the number of follicles with a diameter of >14mm ≤3-4, or the number of eggs acquired ≤3-4, and the peak E2 value before ovulation <300-500pg/ml are considered as ovarian hyporesponsiveness in the conventional stimulation of COH regimen. Ovarian hyporesponsiveness was indicated when one of the following occurred during superovulation: 1. total Gn dosage ≥44; 2. average daily Gn dosage ≥300 U; 3. Gn stimulation cycle ≥15 days. Also indicates ovarian hyporesponsiveness.