1. Severe oligozoospermia, weak and abnormal spermatozoa. (1) Extreme oligozoospermia: sperm density in one-time ejaculate ≤1×106/ml. (2) Extreme weak spermatozoa: a+b ≤1%. (3) Extremely severe dyszoospermia: normal spermatozoa rate ≤1%. (4) Moderate oligo-weak teratozoospermia: sperm density 5-10×106/ml, a+b grade sperm 10-30%, normal morphology sperm rate 5-10%, and live sperm count ≤1×106 after treatment. (5) Severe oligo-weak teratozoospermia: sperm density 1-5×106/ml, a+b grade sperm 1-10%, normal morphology sperm rate 1-5%, and live sperm count ≤1× after treatment. 106 . 2, Obstructive, non-obstructive azoospermia: epididymal or testicular spermatozoa obtained by surgical puncture (excluding genetic defects). 3.Immune infertility: the patient is still infertile after more than 3 cycles of IUI treatment, or the fertilization rate of IVF treatment is less than 30%. 4.In vitro fertilization failure: IVF fertilization rate is less than 30% or no fertilization. 5.Abnormal sperm acrosome: such as round head spermatosis.