Scope: 1. Differential diagnosis of respiratory diseases (COPD, lung cancer, etc.) and tuberculosis; 2. Adjuvant diagnosis of bacillus-negative tuberculosis; 3. Differential diagnosis of pleural/pericardial effusion; 4. Tuberculosis screening for patients with chest shadow to be investigated; 5. Tuberculosis screening for special populations (silicosis, etc.); 6. Tuberculosis infection screening for patients with unexplained fever; 7. Screening for tuberculosis infection in BCG vaccinated population, screening for tuberculosis in HIV infected patients; 8. peritonitis), etc. Clinical applications: 1. diagnosis and differential diagnosis of tuberculosis, evaluation of the efficacy of anti-tuberculosis treatment; 2. tuberculosis diagnosis/infection screening in immunocompromised/suppressed patients; tuberculosis infection screening in patients using biologics or immunosuppressive agents: 3. HIV-infected patients/renal dialysis patients/organ transplant patients; 4. ancillary diagnosis of tuberculosis in children; 5. Screening for TB infection in close contacts/high risk groups; 6. Close contact tracing, etc.