Nucleic acid testing is often used as a basis for ruling out neoplastic pneumonia. A negative nucleic acid test means that the patient is not infected with neoplastic virus, and the incubation period for neoplastic pneumonia is currently 1-14 days. If the body is in a period of disease attack, the body’s immunity is low may affect the nucleic acid test results, false negative, such patients are recommended to recover from the disease before testing. If vomiting or other nasopharyngeal secretions occur during the nucleic acid test, this may also affect the results of the nucleic acid test and should be retested. If the sampling process is not standardized and the sample is contaminated or the machine used to test the sample malfunctions, this may affect the results of the nucleic acid test and should be resampled or re-tested after the machine is repaired. Nucleic acid results are sometimes affected by a variety of factors, so a negative nucleic acid result cannot absolutely rule out neoconiosis, and self-isolation and observation should be done in ordinary life.