Before treatment: 1. Know what kind of wrinkles you have: static wrinkles or dynamic wrinkles, usually Botox purified protein (BOTOX) for dynamic wrinkles (including crow’s feet, head lines/forehead lines are more effective!) Bi Xiaoping, Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine 2. Stop using aspirin, ibuprofen, vitamin E, and other supplements with similar effects such as ginkgo and ginseng 3. Abstain from alcohol to reduce the intake of spicy and stimulating foods; 4. If possible, do not put on makeup or wash your face before the visit.