Urobilinogen is an important urine test that is clinically important for disease diagnosis. So how do we check for an increase in urobilinogen? 1, urine must be fresh and protected from light, otherwise urobilinogen can be oxidized to urobilinogen and show false negative results. 2, Ehrlich method: the reagent and some endogenous substances produce color reaction, such as porphyrin, indole compounds, etc. to produce red, and fecal odorant to produce blue, and some drugs such as sulfonamides and p-aminosalicylates to produce yellow, these are easy to make the test results appear false positive. 3, urine containing a large amount of vitamin C or the use of broad-spectrum antibiotics (inhibited the intestinal flora, so that the urinary bilirubinogen reduction) can appear false negative. 4, urinary bilirubin detection and urinary bilirubin, as well as clinical jaundice can be used as a laboratory indicator to identify, but also with serum bilirubin, fecal bilirubin and other detection indicators together with a comprehensive analysis. 5, the response of the test strip with the increase in temperature and enhance the response of the optimal temperature of 22 ~ 26 ℃. 6, normal human urinary bilirubin excretion fluctuates greatly from day to day, the amount is small at night and in the morning, and increases rapidly in the afternoon, reaching a peak in the afternoon 2-4h; at the same time, the clearance rate of urinary bilirubin is related to the pH of urine, so the test results of urinary bilirubin should be analyzed comprehensively. It has very important clinical significance for disease diagnosis.